PMC

(A) Normal type C₁(H). (B) Pale red egg (re^p-1) mutant. (C) Red egg (re) mutant. (D) Normal type C₁(H) after 36h of oviposition. (E) Pale red egg (re^p-1) mutant after 36h of oviposition. Finally, the eggs of normal type C₁(H) was black colors, the eggs of re^p-1 mutant were pale red colors, the eggs of re mutant was red colors.

(A) Wild-type silkworm variety Fuyin. The eggs are yellow when they are laid and then turn gray after 72 h due to diapause. (B) Fuyin-lre, red egg mutant of Fuyin. Some eggs turn gray and the other eggs turn red 72 h after egg laying. The gray eggs can hatch after incubation, but red eggs cannot hatch. (C) Diapause-free silkworm variety Nistari. The eggs are yellow during the whole egg period. The eggs laid by Fuyin are diapause eggs, and they need to be treated with hydrochloric acid to terminate the diapause. Silkworm eggs after injection are fragile, and hydrochloric acid treatment will cause the death of the eggs. The eggs laid by Nistari are diapause-free eggs and are widely used in microinjection experiments. Diapause-free eggs can develop normally without hydrochloric acid treatment. Therefore, the diapause-free eggs laid by Nistari were used in RNAi.

(A) Structure map of the transgenic vector. (B) Fluorescent images of the transgenic silkworm in egg (a,b) and moth (c,d) stages, the scale bar represents 2 mm. (C) Immunohistochemical analysis of MSG cross sections of transgenic silkworm (a–c) and non-transgenic silkworm (d–f). The green fluorescence represents the immunoblot signals of haFGF proteins; the DAPI stained by blue fluorescence represents the cell nucleus. Scale bar represents 500 μm. (D) Immunohistochemical analysis of raw silk cross sections of transgenic silkworm (a,b) and non-transgenic silkworm (c,d). Scale bar represents 10 μm. (E) SDS–PAGE analysis of the cocoon proteins from ten different transgenic lines; the percentages represent the haFGF content of each line in the total cocoon extracts. (F) Western blot analysis of the haFGF in cocoon extracts from ten different transgenic lines.

Generation of the PDGF-BB transgenic silkworm. (A) Structural map of the transgenic vector. 3xp3EGFP represents the selection marker of the transgene; hr3CQ represents an enhancer isolated from the Bombyx mori nuclear polyhedrosis virus (BmNPV) genome of a Chongqing stain; Ser1 Pro represents the promoter region of the sericin-1 gene; SP indicates the signal peptide of the sericin-1 gene; Ser1PA indicates the polyA sequence of the sericin-1 gene. (B) White light image of a transgenic silkworm egg. (C) Fluorescence image of a transgenic silkworm egg. (D) White light image of a wild type silkworm moth. (E) Fluorescence image of a wild type silkworm moth. (F) White light image of a transgenic silkworm moth. (G) Fluorescence image of a transgenic silkworm moth. Scale bars are 2 mm.